Epithelial junctions, cytoskeleton, and polarity, by Gabriella Pásti and Michel Labouesse (an update of Epithelial junctions and attachments, by Michel Labouesse), is now available in the Cell biology section of WormBook. This chapter discusses the composition, assembly and remodeling of C. elegans cell-cell and hemidesmosome-like cell-extracellular matrix junctions, proteins that anchor the cytoskeleton, and mechanisms involved in establishing epithelial polarity.

Overview of gene structure in C. elegans, by John Spieth, Daniel Lawson, Paul Davis, Gary Williams, and Kevin Howe (an update of Overview of gene structure, by Spieth and Lawson), has just been added to the Genetics and genomics and Molecular biology sections. This chapter describes some of the ways in which gene structures are currently curated in WormBase, primarily focusing on transcribed regions of genes, and also includes an updated description of non-coding RNA genes.

Volume 20, Number 2 of The Worm Breeder's Gazette has been published. This issue highlights genome editing and includes 4 articles describing improvements in CRISPR/Cas9 technology, web resources useful for those experiments, and links to published papers in the field. The issue also includes descriptions of other useful tools for the worm community, a caution about the potential effects of bacto-peptone on worm behavior, and several other research summaries. There are also worm humor articles, worm art, and new lab announcements.

Development and migration of the C. elegans Q neuroblasts and their descendants by Teije Middelkoop and Rik Korswagen has been published. This chapter gives an overview of what is currently known about the mechanisms that mediate and control the development and migration of the Q neuroblasts and their descendants.

Assaying mechanosensation, by Martin Chalfie, Anne Hart, Catharine Rankin, and Miriam Goodman, has been published in the Behavioral assays section of WormMethods. In addition to updating and expanding assays for a variety of touch responses, the new chapter describes mechanical stimulation during electrical or optical recording.

Mass spectrometry-based shotgun proteomic analysis of C. elegans protein complexes, by Bryan Fonslow, James Moresco, Patricia Tu, Antti Aalto, Amy Pasquinelli, Andrew Dillin and John Yates, has been published in the Cell and molecular biology section of WormMehtods. This chapter describes the use of mass spectrometry-based shotgun proteomics coupled with co-immunoprecipitation to discover new interactions among proteins.